Figure 3.
Microscope images of CD8+ T cell lines
HLA-A∗02:01 restricted M158-specific CD8+ T cells were expanded from a HLA-A∗02:01+ individual as per the protocol described in this manuscript. Images were taken on a Nikon Eclipse Ti microscope on a ×4,×10 and ×20 objective daily from Day 1 to Day 10.
(A) Representative photo depicting cells that are too sparse, which can limit interactions between cells which can prevent adequate activation.
(B) Images demonstrating how cells sometimes preferentially clump around the edges of tissue culture wells, therefore it is important to resuspend and redistribute cells during media changes.
(C) Representative image of round clumps which can be indicative of peptide-specific proliferation. Scale bars depict 100 μm at the various magnifications.