Supplimentum Completum (SC)

Reagent	Final concentration	Amount
RPMI-1640	N/A	400 mL
100× MEM Non-essential Amino Acids	20% (v/v)	100 mL
L-Glutamine	0.6% (w/v)	3 g
HEPES	2% (w/v)	11.9 g
β-mercaptoethanol	0.007% (v/v)	35 μL
Total	N/A	500 mL

Prepare 30 mL aliquots and store at −20°C until use.