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R0 media
| Reagent | Final concentration | Amount |
|---|---|---|
| RPMI-1640 | N/A | 500 mL |
| SC | 5% (v/v) | 30 mL |
| 100× Penicilin/Streptimycin/Gentamycin | 1% (v/v) | 5 mL |
| Total | N/A | 535 mL |
Store at 4°C ideally for no longer than a month. Discard if media turns bright pink due to alkalization caused by aeration. Media should be clear, discard if media becomes cloudy as it is suggestive of contamination.
