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. 2022 Jul 9;25(8):104730. doi: 10.1016/j.isci.2022.104730

Figure 2.

Figure 2

Assessing epithelial polarity reversibility in organoids transitioned from suspension to Matrigel-embedded culture

(A) Human ABSC aggregates were formed in ECM-free suspension culture for 1 day and then transferred into ECM-rich, Matrigel-embedded culture and maintained for an additional 20 days.

(B) Immunofluorescence images of day 21 organoids stained for FOXJ1, Ac-α-Tub, and ZO-1. Scale bar, 25 μm.

(C) Quantification of the percentage of day 21 (D21) organoids with apical-out versus apical-in epithelial polarity indicated by apical Ac-α-Tub localization.

(D and E) Quantification of FOXJ1+ cell abundance (D) and percentage ciliation (E) in day 21 (D21) organoids. Data represent means ± SD from ≥15 organoids. ∗∗∗p < 0.001, Unpaired t-test.