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. 2022 Jul 22;13:921728. doi: 10.3389/fimmu.2022.921728

Figure 4.

Figure 4

Gene expressions after PolyIC–WD–TNF at different concentrations of Poly (I:C) and TNFα. MRC-5 cells were initially treated with Poly (I:C) for 24 h and were then washed and rested for 24 h before being secondarily treated with TNFα for another 24 h. (A–E) Effect of different concentrations of Poly (I:C) (0, 1, 10 µg/ml) and TNFα (0, 1, 5, 10 ng/ml) on gene expressions of IL1B, IL6, IL8, MMP8, and MMP9. Secondary treatment to TNFα dose-dependently induced these gene expressions. Preexposure to Poly (I:C) did not affect TNFα-induced IL1B, IL8, MMP8, and MMP9 gene expressions [PolyIC (0 µg/ml)–WD–TNF (10 ng/ml) vs. PolyIC (10 µg/ml)–WD–TNF (10 ng/ml)]. (F) Preexposure to Poly (I:C) (10 µg/ml) and secondary treatment of MRC-5 cells with TNFα (10 ng/mL) did not affect the fibrosis-related genes; ACTA2, COL1A1, POSTN, and TGFB1. N = 4. Values with * were defined significant when compared to (-)-WD-(-) while *above bar were significant between two variables.