Fig. 3.

Effects of flavonols on intracellular ROS in LPS-induced RAW264.7 cells. Cells were pretreated with flavonols for 4 h and induced with LPS (1 μg/ml) for 12 h, then they were incubated with DCFH-DA. Positive cells were (A, B) counted by cell flow cytometry and (C) visualized by Leica DMI8 fluorescence. Values were mean ± SD. *P < 0.05 and ****P < 0.0001 versus LPS, ^####P < 0.0001 versus Control.