Fig. 7.

IR-induced iron overload disrupted mitochondrial iron homeostasis. (A) Representative images of Mito-FerroGreen-stained control or DFO (500 nM)-treated HIEC cells after 0 or 6 Gy IR showing mitochondrial Fe²⁺. The nuclei were counterstained with Hoechst 33342. Scale bar = 10 μm. (B) Bar graph showing fluorescence intensity in the indicated cells. n = 3 independent experiments. (C) Representative images of MitoSox-stained control or DFO (500 nM)-treated HIEC cells after 0 or 6 Gy IR showing mitochondrial ROS generation. The nuclei were counterstained with Hoechst 33342. Scale bar = 50 μm. (D) Bar graph showing fluorescence intensity in the indicated cells. n = 3 independent experiments. Mfrn2 (E) and Ftmt (F) mRNA expression in control or DFO (500 nM)-treated HIEC cells after 0 or 6 Gy IR. n = 3 independent experiments. (G) Mfrn2 mRNA levels in siNC or siMfrn2 HIEC cells exposed to IR (6 Gy). (H) Representative images of Mito-FerroGreen-stained siNC or siMfrn2-treated HIEC cells after 0 or 6 Gy IR showing mitochondrial Fe²⁺. The nuclei were counterstained with Hoechst 33342. Scale bar = 10 μm. (I) Bar graph showing fluorescence intensity in the indicated cells. n = 3 independent experiments. (J) Representative images of MitoSox-stained siNC or siMfrn2-treated HIEC cells after 0 or 6 Gy IR showing mitochondrial ROS generation. The nuclei were counterstained with Hoechst 33342. Scale bar = 50 μm. (K) Bar graph showing fluorescence intensity in the indicated cells. n = 3 independent experiments. All data are presented as mean ± S.D. ns, p ≥ 0.05; ∗, p < 0.05; ∗∗, p < 0.01; ∗∗∗, p < 0.001.