FIG. 1.

Regulated expression of CooD from the ParaBAD promoter. Protein extracts were prepared from E. coli LMG194/pEU1290/pEU1206 grown in the presence of 0.2% glucose (wt/vol) (lane 2) or in the presence of arabinose at 0.002% (lane 3), 0.01% (lane 4), and 0.05% (lane 5) and were analyzed by immunoblotting with anti-CooD antiserum. Lane 1 contains a negative-control extract from LMG194/pEU1290. Lane 6 contains protein markers with molecular masses indicated in kilodaltons. The positions of the full-length, 38-kDa form of CooD and the 25-kDa truncated form (CooD*) are indicated by arrows.