TABLE 1.
Effect of modulating CooD expression on the number of CS1 pili produced per cell in E. coli LMG194/pEU1206
| Cell no. | No. of pili/cella
|
|||
|---|---|---|---|---|
| Glucoseb (0.20%) | Arabinose
|
|||
| 0.002% | 0.01% | 0.05% | ||
| 1 | 11 | 4 | 0 | 12 |
| 2 | 11 | 12 | 3 | 47 |
| 3 | 14 | 21 | 4 | 60 |
| 4 | 16 | 23 | 6 | 64 |
| 5 | 16 | 129 | 20 | 75 |
| 6 | 19 | 134 | 64 | 85 |
| 7 | 20 | >150 | 97 | 100 |
| 8 | 21 | >150 | 110 | 105 |
| 9 | 22 | >150 | 126 | >150 |
| 10 | 23 | >150 | >150 | >150 |
| 11 | 26 | >150 | >150 | >150 |
| 12 | 26 | >150 | >150 | >150 |
| 13 | 26 | >150 | >150 | >150 |
| 14 | 26 | >150 | >150 | >150 |
| 15 | 26 | >150 | >150 | >150 |
| 16 | 27 | >150 | >150 | >150 |
| 17 | 27 | >150 | >150 | >150 |
| 18 | 28 | >150 | >150 | >150 |
| 19 | 28 | >150 | >150 | >150 |
| 20 | 34 | >150 | >150 | >150 |
| 21 | 39 | >150 | >150 | >150 |
| 22 | 41 | >150 | >150 | >150 |
| 23 | 45 | >150 | >150 | >150 |
| 24 | 53 | >150 | >150 | >150 |
| 25 | 82 | >150 | >150 | >150 |
a
The number of pili per cell was determined for 25 bacteria grown in the presence of glucose or arabinose.
b
When the cells were grown with glucose, the mean number of pili per cell was 28.3, with a standard deviation of 15.1 and standard error of 3 (n = 25).