Fig. 3.

Higher myogenic capacity of PDGFRβ lineage cells from young mice. A Myotube formation of MPCs isolated from young and old mice. B Myotube formation of myoblasts was assessed by immunofluorescence staining of MyHCf. Staining (purple) shows GFP + myotubes (green) were MyHCf + in young mice, and MyHC + myotubes in old mice were GFP-. Nuclei were stained with DAPI. C Quantification of MyHC-f and GFP both positive myotubes (n = 6). The percentage of differentiated myotubes was quantified as the number of nuclei in MyHC-f positive myotubes relative to the total number of nuclei. Error bars indicate ‘mean ± SD,’ *p < 0.05. Scale bars = 50 µm