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Auxotrophic complementation test of T. thermophilus lysine mutants. Tubes: 1, MP medium; 2, MP medium plus 1 mM lysine; 3, MP medium plus 1 mM diaminopimelic acid; 4, MP medium plus 1 mM α-aminoadipic acid. Mutant K1001, which could not grow in a minimal medium even after addition of 1 mM α-aminoadipic acid, and mutant K1003, which grew after addition of 1 mM α-aminoadipic acid, are shown as examples. Auxotrophy of K1002 and K1004 was the same as that of K1001 and K1003, respectively.
