Skip to main content
. Author manuscript; available in PMC: 2023 Apr 19.
Published in final edited form as: Anal Chem. 2022 Apr 6;94(15):6017–6025. doi: 10.1021/acs.analchem.2c00646

Figure 1.

Figure 1.

Diagram of the dual-column nanoLC system. The system interweaves two valves and employs two subsystems, each consisting of a sample loop, SPE column and analytical column. These subsystems share a sample collection tip, syringe, gradient LC pump and isocratic loading pump, allowing for SPE loading and column washing on one subsystem, while the other elutes peptides. Shown on the left is a commercial LC module containing both a gradient and a loading pump, which provides flow for all operations.