Impact of cycle time on proteome coverage. (A) Protein groups identified with or without MBR for different cycle times. Error bars represent standard deviations (n = 10). (B) Distribution of peptides identified across the cycle. Duty cycles were 58, 69, 73, and 83% for 7, 10, 15, and 30 min cycles, respectively. (C) Proteome coverage across 250 replicate injections of 200 pg aliquots of HeLa tryptic digest.