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. Author manuscript; available in PMC: 2023 Apr 19.
Published in final edited form as: Anal Chem. 2022 Apr 6;94(15):6017–6025. doi: 10.1021/acs.analchem.2c00646

Figure 4.

Figure 4.

Impact of cycle time on proteome coverage. (A) Protein groups identified with or without MBR for different cycle times. Error bars represent standard deviations (n = 10). (B) Distribution of peptides identified across the cycle. Duty cycles were 58, 69, 73, and 83% for 7, 10, 15, and 30 min cycles, respectively. (C) Proteome coverage across 250 replicate injections of 200 pg aliquots of HeLa tryptic digest.