TABLE 3.
β-Galactosidase level in B. subtilis wild-type and deoR strains carrying different dra-lacZ fusionsa
| Strain | Region fused to lacZ | deoR status | CRE status | Inducer added | Enzyme activity (nmol/min/mg)b |
|---|---|---|---|---|---|
| XM15 | −80–+154 | + | + | None | 15 |
| dRib | 262 | ||||
| XM25 | −80–+154 | − | + | None | 107 |
| dRib | 110 | ||||
| XM16 | −80–+60 | + | − | None | 138 |
| dRib | 1,465 | ||||
| XM26 | −80–+60 | − | − | None | 1,133 |
| dRib | 1,029 |
a
Cells were grown in succinate minimal medium. Inducer was added to a final concentration of 1 mg/ml.
b
The enzyme levels of XM15 and XM16 were taken from Fig. 2.