Figure 3.

ANTXR1 activates the Wnt/β-catenin signaling pathway by promoting β-catenin into the nucleus. (a) ANTXR1 interacted with LRP6 shown by the Co-IP assay that the lysates from the K562 cells transfected with the ANTXR1–GFP and LRP6-Flag plasmids by using GFP- and Flag-targeting antibodies. (b) β-Catenin expression in the cytoplasm and nucleus was detected by western blot analysis after K562 cells were transfected with the ANTXR1 vector, control vector, ANTXR1-shRNA vector, or NC-shRNA vector. (c, d) Quantification of western blots. The error bar represents the SD.^∗P < 0.05 and ^∗∗P < 0.01. NC designates the negative control.