Figure 3.

The miR-147a inhibitor prevents ferroptosis of human glioblastoma cells. (a) miR-147a level in human glioblastoma cells treated with or without the miR-147a inhibitor. (b) Cell viability detected by CCK-8 assay. (c) LDH level in the medium from cells treated with or without the miR-147a inhibitor. (d) ROS level detected by DCFH-DA probe. (e) MDA level in cells treated with or without the miR-147a inhibitor. (f) Relative level of intracellular iron. (g–h) Quantification of mtDNA content and integrity. (i) Quantification of intracellular ATP level. N = 6 for each group. Data were expressed as the mean ± SD, and P < 0.05 was considered statistically significant.