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Southern blot analysis of Anabaena sp. strain PCC 7120 crh genes. Southern blots of digested Anabaena genomic DNA (3 μg) were hybridized at 60°C with either the crhC (A) or the crhB (B) PCR fragment. The autoradiograms are shown. Restriction enzymes utilized were ClaI (lanes 1), EcoRI (lanes 2), HindIII (lanes 3), and XbaI (lanes 4). The positions of lambda HindIII molecular mass markers (kilobases) are shown to the left.
