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. 2022 Apr 12;20(8):1284–1294. doi: 10.1158/1541-7786.MCR-21-0905

Figure 6.

Figure 6. C9b cooperates with KRAS activation driving lung tumorigenesis. KRASG12D L/+ mice with intratracheal injection (B) of lentiviral particles containing GFP-, WT-, or Mut C9b-Cre(T2a)Luc (A) developed lung tumors (C) at 32 weeks post injection. IHC staining (C) with indicated antibodies shows the expression of Myc-tagged WT or mutant C9b, ERK activation (pERK), and HMGA2 in tumors and the presence of Gr1+ cells in the lung (black arrowheads). Orange arrows point to the clusters of immune cells observed. The percent replacement of normal lung parenchyma by tumor cells based on H&E and pERK signal (n = 12 for GFP, 10 for WT C9b, and 11 for Mut C9b, D), the HMGA2 positivity score (E), and the number of Gr1+ cells per view (20X magnification, G) (n = 4 for GFP, 6 for WT C9b and Mut C9b, average of 3 views per mouse for E and G) in each group are shown. F, Metastasis observed in the liver (H&E, 10x magnification) in a mouse with WT C9b expression. Data in D, E and G are means ± SEM combined from 2 independent intratracheal injection experiments. Adjusted P values are determined by ANOVA Tukey multiple comparison test.

C9b cooperates with KRAS activation driving lung tumorigenesis. KRASG12D L/+ mice with intratracheal injection (B) of lentiviral particles containing GFP-, WT-, or Mut C9b-Cre(T2a)Luc (A) developed lung tumors (C) at 32 weeks post injection. IHC staining (C) with indicated antibodies shows the expression of Myc-tagged WT or mutant C9b, ERK activation (pERK), and HMGA2 in tumors and the presence of Gr1+ cells in the lung (black arrowheads). Orange arrows point to the clusters of immune cells observed. The percent replacement of normal lung parenchyma by tumor cells based on H&E and pERK signal (n = 12 for GFP, 10 for WT C9b, and 11 for Mut C9b, D), the HMGA2 positivity score (E), and the number of Gr1+ cells per view (20X magnification, G; n = 4 for GFP, 6 for WT C9b and Mut C9b, average of 3 views per mouse for E and G) in each group are shown. F, Metastasis observed in the liver (H&E, 10x magnification) in a mouse with WT C9b expression. Data in D, E and G are means ± SEM combined from 2 independent intratracheal injection experiments. Adjusted P values are determined by ANOVA Tukey multiple comparison test.