KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| SLC25A37 | Invitrogen | Cat#PA5-26720; RRID:AB_2544220 |
| SLCO2B1 | Novus Biologicals | Cat#NBP1-80979; RRID:AB_11038565 |
| Fth1 | Cell Signaling Technology | Cat#3998S; RRID:AB_1903974 |
| IRP2 | Cell Signaling Technology | Cat#37135S; RRID:AB_2799110 |
| β-Actin | GeneTex | Cat#GTX109639; RRID:AB_1949572 |
| GAPDH | GeneTex | Cat#GTX627408; RRID:AB_11174761 |
| HMOX2 | Proteintech | Cat#14817-1-AP; RRID:AB_2118843 |
| UROD | Proteintech | Cat#15547-1-AP; RRID:AB_2213813 |
| ALAS1 | Proteintech | Cat#16200-1-AP; RRID:AB_2878227 |
| CRISPR-Cas9 | Novus Biologicals | Cat#NBP2-36440; Clone 7A9-3A3 |
| TFRC (CD71) | Cell Signaling Technology | Cat#13113S; RRID:AB_2715594 |
| GFP (for immunofluorescence) | Aves Labs | Cat#GFP-1010; RRID:AB_2307313 |
| GFP (for immunoblotting) | Proteintech | Cat#50430-2-AP; RRID:AB_11042881 |
| Goat anti-Chicken IgY (H+L), Alexa Fluor™ 488 | Thermo Fisher Scientific | Cat#A11039; RRID:AB_2534096 |
| Goat anti-Rabbit IgG (H+L), Alexa Fluor™ 555 | Thermo Fisher Scientific | Cat#A21428; RRID:AB_2535849 |
| PE/Cy7 anti-mouse CX3CR1 [Clone: SA011F11] | Biolegend | Cat#149015; RRID:AB_2565699 |
| Anti-CD11b Rat Monoclonal Antibody (Brilliant Violet® 711) [clone: M1/70 | Biolegend | Cat#101241; RRID:AB_11218791 |
| Anti-Ly-6C Rat Monoclonal Antibody (Brilliant Violet® 510) [clone: HK1.4] | Biolegend | Cat#128033; RRID:AB_2562351 |
| Bacterial and Virus Strains | ||
| NEB Stable Competent E. coli | New England Biolabs | C3040 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| RPMI 1640 Culture Media | GIBCO | 11875 |
| DMEM Culture Media | GIBCO | 11965 |
| Trypsin | GIBCO | 25200 |
| Fetal Bovine Serum | Sigma | 12306C |
| Bafilomycin A1 | Cayman Chemical | 11038 |
| Concanamycin A | Sigma-Aldrich | C9705 |
| Deferoxamine Mesylate | Sigma | D9533 |
| Deferiprone | Selleck Chemicals | S4067 |
| Antimycin A | Sigma-Aldrich | A8674 |
| CB-839 | Selleck Chemicals | S7655 |
| Palmitic Acid | Cayman Chemical | 10006627 |
| L-Buthionine-sulfoximine | Sigma | B2515 |
| DMSO | AlfaAesar | A13280-36 |
| HPLC Grade Water | Fisher Scientific | W6 |
| HPLC Grade Methanol | Fisher Scientific | A454-1 |
| Heavy labeled amino acid internal standards | Cambridge Isotopes | MSK-A2-1.2 |
| Ammonium Iron (III) Citrate (FAC) | Sigma | F5879 |
| Ammonium Chloride | Sigma | A9434 |
| Zn(II) Mesoporphyrin IX | Frontier Scientific | M40628 |
| Hemin | Sigma-Aldrich | H9039 |
| Succinylacetone | Cayman Chemical | 25501 |
| Ethanol | Fisher Scientific | 04-355-222 |
| BsmBI | New England Biolabs | R0580 |
| T4 DNA ligase | New England Biolabs | M0202 |
| ExTaq DNA Polymerase | Takara | RR001A |
| Hank's Buffered Salt Solution | GIBCO | 24020117 |
| XtremeGene9 | Roche | 6365779001 |
| Polybrene | Sigma | H9268 |
| Puromycin | Sigma | P8833 |
| Blasticidin | Invivogen | ant-bl-1 |
| Critical Commercial Assays | ||
| CellTiter-Glo® Luminescent Cell Viability Assay | Promega | G7571 |
| Deposited Data | ||
| Original western blot images | This work | https://doi.org/10.17632/sg3b3f25p4.1 |
| Experimental Models: Cell Lines | ||
| Jurkat | ATCC | TIB-152 |
| HepG2 | ATCC | HB-8065 |
| MDA-MB-231 | ATCC | CRM-HTB-26 |
| HEK293T | ATCC | CRL-1573 |
| PaTu-8988t | Dr. Monther Abu-Remaileh (Stanford University) | N/A |
| AK196 | Dr. Haoqiang Yang (MD Anderson Cancer Center) | N/A |
| HY15549 | Dr. Nabeel El-Bardeesy (Massachusetts General Hospital Cancer Center) | N/A |
| 4T1 | Dr. Sohail Tavazoie (Rockefeller University) | N/A |
| 4T07 | Dr. Sohail Tavazoie (Rockefeller University) | N/A |
| Experimental Models: Organisms/Strains | ||
| C57BL/6N-Slco2b1tm1a(KOMP)Wtsi/Mmucd | Mutant Mouse Resource and Research Center (MMRRC) | 049765-UCD |
| C57BL/6J | The Jackson Laboratory | 000664 |
| Oligonucleotides | ||
| DNA oligonucleotides and primers | This study | See Table S19 |
| Recombinant DNA | ||
| pLV-EF1a-IRES-Blast | Addgene | 85133 |
| plentiCRISPR puro v2 | Addgene | 52961 |
| lentiGuide Puro | Addgene | 52963 |
| CRISPRa dCas9-VPR vector with hEF1a promoter | Horizon Discovery | CAS11916 |
| pLV-EF1a-IRES-Blast_SLC25A37 | This study | N/A |
| pLV-EF1a-IRES-Blast_SLCO2B1 | This study | N/A |
| pLV-EF1a-IRES-Blast_SLCO2B1 (short isoform) | This study | N/A |
| pLV-EF1a-IRES-Blast_SLCO2B1-eGFP | This study | N/A |
| pLV-EF1a-IRES-Blast_SLCO2A1 | This study | N/A |
| pLV-EF1a-IRES-Blast_SLCO2A1-eGFP | This study | N/A |
| pLV-EF1a-IRES-Blast_Slco2b1 (mouse cDNA) | This study | N/A |
| plentiCRISPR puro v2_sgTFRC_1 | This study | N/A |
| plentiCRISPR puro v2_sgUROD_1 | This study | N/A |
| plentiCRISPR puro v2_sgUROD_2 | This study | N/A |
| plentiCRISPR puro v2_sgHMOX2_1 | This study | N/A |
| Human metabolism-focused CRISPRa sgRNA library | This study | N/A |
| Mouse metabolism-focused CRISPRa sgRNA library | This study | N/A |
| Metabolism-focused sgRNA library | Birsoy et al., 2015 | N/A |
| Software and Algorithms | ||
| XCaliburQual Browser 2.2 | ThermoFisher | https://www.thermofisher.com/order/catalog/product/OPTON-30965#/OPTON-30965; RRID: SCR_014593 |
| Skyline Daily | MacCoss Lab | https://skyline.ms/project/home/software/Skyline/begin.view; RRID: SCR_014080 |
| FCS Express 7 | DeNovo Software | https://denovosoftware.com/; RRID: SCR_016431 |
| Prism 9 | GraphPad | https://www.graphpad.com/scientific-software/prism/; RRID: SCR_002798 |
| Other | ||
| Z2 Coulter Counter | Beckman | Model Z2 |
| SpectraMax Microplate Reader | Molecular Devices | Model M5 |
| Primovert Microscpe | Carl Zeiss | 415510-1105-000 |
| FACSAriaII | BD Biosciences | 643181 |
| Multiphoton microscope with confocal modality | Nikon | A1R MP |