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. 2022 Aug 6;13:4580. doi: 10.1038/s41467-022-32284-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Graphical representation of the experimental setup. Experimental MI was induced in mice by the permanent ligation of the proximal portion of the left anterior descending artery (LAD). Samples were isolated from mouse hearts at days 0, 1, 3, 5, and 7 post-MI. Tissues were enzymatically digested and live CD45⁺ cells were FACS-sorted and loaded for scRNA-seq. b Two-dimensional uniform manifold approximation and projection (UMAP) visualization of the 33,977 cardiac CD45⁺ cells identified 12 broad cell types after unsupervised clustering. Each point represents a single cell; cell types are color-coded. c Left: dot plot of well-known cell-type-specific marker genes per cell type. The dot intensity (from white to red) represents the average expression value of all cells per cell type and the dot size represents the proportion of cells expressing the genes. Right: feature plot representing the expression levels of the selected cell-type-specific marker genes. d Bar plot representing the proportions of cells in each of the 12 broad cell types according to the time-point after MI. Source data are provided in the Source Data file.