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. 2022 Aug 6;13:4587. doi: 10.1038/s41467-022-32255-7

Fig. 9. Collagen XII overexpression promotes metastasis in vivo.

Fig. 9

a Bulk modulus as determined by unconfined compression analysis of excised primary tumours generated by co-implantation of cancer cells with control (eGFP-VPR) and collagen XII overexpressing (ColXII-VPR1, ColXII-VPR2) CAFs. Data are presented as mean ± SD, n = 7 eGFP-VPR mice, n = 7 ColXII-VPR1 and n = 8 ColXII-VPR2 mice, *p = 0.017, **p = 0.0066, Kruskal–Wallis test with Dunn’s multiple comparisons test. b Quantification of mean number of metastases normalised to the total lung area in mice with metastases present in the overexpression study (n = 6 eGFP-VPR mice, n = 6 ColXII-VPR1 and n = 4 ColXII-VPR2 mice with metastases present). Data are presented as mean ± SD. eGFP-VPR vs. ColXII-VPR1 p = 0.0087; eGFP-VPR vs. ColXII-VPR2 p = 0.0048, two-sided Mann–Whitney U-test. c Quantification of collagen XII-positive area in primary tumours from the overexpression study. n = 7 eGFP-VPR mice, n = 7 ColXII-VPR1 and n = 8 ColXII-VPR2 mice. **p < 0.01, eGFP-VPR vs. ColXII-VPR1 p = 0.0098; eGFP-VPR vs ColXII-VPR2 p = 0.025, two-sided Mann–Whitney U-test. One-way ANOVA with a Dunnett’s multiple comparisons test. Data are presented as mean ± SD. d Quantification of pMLC2 positive area in primary tumours from the overexpression study. n = 7 eGFP-VPR mice, n = 7 ColXII-VPR1 and n = 8 ColXII-VPR2 mice. *p = 0.04, two-sided Mann–Whitney U-test. Data are presented as mean ± SD. Source data are provided in the Source data file.