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. 2022 Aug 6;13(8):687. doi: 10.1038/s41419-022-05117-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A MCF10A and MCF-ErbB2 cells were cultured detached from the ECM (3D) for 24 h and BLNK mRNA levels were analyzed in the cells by quantitative PCR (qPCR). BLNK mRNA levels normalized by those of 18 S rRNA (determined by qPCR) expressed in arbitrary units are shown. Results represent the average of three independent experiments plus the SD. *p-value is < 0.05. B MCF10A and MCF-ErbB2 cells were cultured attached to (2D) or detached from (3D) the ECM for the indicated times and assayed for BLNK levels by western blotting. α-tubulin was used as a loading control. C–E Human ErbB2-positive cell lines AU565, SKBR3 and BT474 were cultured attached to (2D) (left) or detached from the ECM (3D) (right) for 48 h in the absence (−) or in the presence (+) of 5 μg/ml trastuzumab (TZ) and assayed as in (B). F Trastuzumab-resistant variant of BT474 cells BT474TR was assayed as in (C–E).