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. 2022 Aug 6;13(8):687. doi: 10.1038/s41419-022-05117-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A MCF10A cells transfected with 100 nM control RNA (cont RNA) or BLNK-specific siRNA (BLNK siRNA) 7 or 9 were detached for 3 h (3D) and assayed for BLNK expression by western blotting. α-tubulin was used as a loading control. B MCF10A cells treated as in A were allowed to form colonies in monolayer immediately or after being detached for the indicated times. The ratio of colony number formed by the cells detached for each time period to that formed by the cells plated in monolayer immediately after transfection is shown in each case. This ratio observed for the cells detached for 24 h was designated as 1.0. The data represent the average of three independent experiments plus the SD.