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. 2022 Aug 6;13(8):687. doi: 10.1038/s41419-022-05117-9

Fig. 5. BLNK triggers apoptosis of detached ErbB2-overproducing breast cancer cells.

Fig. 5

A–E Indicated cell lines were cultured detached form the ECM in the absence (−) or in the presence (+) of 3 ng/ml doxycycline (doxy) for 24 h and assayed for Annexin V binding and permeability to 7-AAD by flow cytometry. Representative flow cytometry plots are shown in A–D. % Annexin V- and 7AAD-negative (lower left quadrant), Annexin V-positive and 7AAD-negative (upper left quadrant), Annexin V-positive and 7AAD-positive (upper right quadrant) and Annexin V-negative and 7AAD-positive (lower right quadrant) cells in the total cell population is shown. Results of three independent experiments plus SD are shown in (E). % Annexin V-positive/7-AAD positive cells in E is the sum of the percentages of Annexin V-positive/7AAD-negative, Annexin V-positive/7AAD-positive and Annexin V-negative/7AAD-positive cells. *p-value < 0.05. F Indicated cell lines were cultured detached form the ECM in the absence (−) or in the presence (+) of 3 ng/ml doxycycline (doxy) for 48 h and assayed for caspase-3 expression. α-tubulin was used as a loading control.