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. 2022 Aug 6;13(8):687. doi: 10.1038/s41419-022-05117-9

Fig. 7. Bortezomib causes BLNK-dependent anoikis of ErbB2-overproducing breast cancer cells.

Fig. 7

A, B BT474 cells were cultured detached from the ECM (3D) for 48 h in the absence (−) or in the presence (+) of 100 nM bortezomib (BZ) and assayed for IRF6 (A), BLNK (B) and phospho-p38MAPK (C) expression by western blotting. GAPDH was used as a loading control in (A, B) and total p38MAPK, in C. D BT474 cells were infected with a control lentivirus or that encoding BLNK shRNA (BLNKshRNA) 7 or 8, cultured for 24 h in the absence (−) or in the presence (+) of 100 nM bortezomib (BZ) detached from the ECM (3D) and assayed for BLNK expression by western blotting. α-tubulin was used as a loading control. E BT474 cells were treated as in C, cultured detached from the ECM (3D) for 120 h in the absence (−) or in the presence (+) of 100 nM bortezomib (BZ) and counted. The number of control cells was designated as 100%. The data are the average of the three independent experiments plus the SD. *p-value < 0.05.