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. 2022 Aug 6;13(8):687. doi: 10.1038/s41419-022-05117-9

Fig. 8. BLNK blocks the ability of ErbB2-positive breast cancer cells to form tumors in mice.

Fig. 8

A Indicated cell lines were cultured detached from the ECM (3D) for 24 h in the absence (−) or in the presence (+) of 500 ng/ml doxycycline (doxy) and assayed for BLNK expression by western blotting. α-tubulin was used as a loading control. B Indicated cell lines were cultured as in A for 120 h in the ″starvation″ medium which represented the Hybri-Care medium supplemented with 0.1% Fetal Bovine Serum and diluted 2.5-fold with HBSS medium and counted. The data are the average of the three independent experiments plus the SD. *p-value < 0.05. C, D Female Nu/Nu Nude mice were injected with the indicated cells in the mammary fat pad (n = 7). 2 mg/ml doxycycline was added or not to the animals’ drinking water. Tumor volumes were measured at the indicated times. The data represent the average tumor volumes plus the SE. One mouse injected with BTLN3-BLNK cells was sacrificed on day 36 due to weight loss, one mouse injected with BTLN3-BLNK cells and receiving doxycycline was sacrificed on day 42 due to a tumor ulcer, one mouse injected with BTLN3-vector cells and receiving doxycycline was sacrificed on day 36 and another one, on day 55 due to diarrhea.