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. Author manuscript; available in PMC: 2023 Feb 5.
Published in final edited form as: Mol Cancer Res. 2022 Aug 5;20(8):1183–1192. doi: 10.1158/1541-7786.MCR-22-0111

Figure 3. PARP inhibition in presence of cisplatin dramatically alters the HeLa cell transcriptome.

Figure 3.

(A) Cells were treated with 10 μM BYK for 30 minutes prior to cisplatin (CDDP) treatment. 2 μM CDDP was added for 6 hours as indicated. DMSO was used as a vehicle control. RNA-seq libraries were prepared following cell collection.

(B) Venn diagram depicting differentially expressed genes from RNA-seq in HeLa cells treated with CDDP (green), BYK (red), or BYK + CDDP (blue).

(C) Heat map representation of the genes that were either up-regulated or down-regulated after treatment of HeLa cells with DMSO (vehicle), CDDP alone, BYK alone, or both.

(D) Box plots showing the fold change in expression for the up-regulated and down-regulated subsets of genes. Boxes marked with different letters are significantly different from each other (Wilcoxon Signed-Rank test; p < 2.2 × 10−16). (E) Gene ontology analysis for BYK + CDDP up-regulated and down-regulated genes. The gene counts in each term and the log10(p-value) for each term are shown.