Table 2.
Conformational transitions of membrane proteins modeled with experimental EPR DEER distance restraints.
| Protein (Organism) | Starting Conformation (PDB ID) | Target Conformation (PDB ID) | No of Restraints | No of helical Residues modeled | References |
|---|---|---|---|---|---|
| Rhodopsin (Bos taurus) | Active (2X72) | Inactive (1GZM) | 10 | 96 | (Li et al., 2004; Standfuss et al., 2011) |
| Rhodopsin | Inactive (1GZM) | Active (2X72) | 10 | 96 | |
| LeuT (Aquifex aeolicus) | Inward-facing (3TT3) | Outward-facing (2A65) | 11 | 167 | (Kazmier et al., 2014a; Krishnamurthy and Gouaux, 2012; Yamashita et al., 2005) |
| vSGLT (Vibrio parahaemolyticus) | Outward-opena (5NV9) | Substrate free (2XQ2) | 11 | 241 | (Paz et al., 2018; Wahlgren et al., 2018; Watanabe et al., 2010) |
| Mhp1 (Microbacterium liquefaciens) | Outward-facing (2JLN) | Inward-facing (2X79) | 14 | 209 | (Shimamura et al., 2010; Weyand et al., 2008) |
| Mhp1 | Inward-facing (2X79) | Outward-facing (2JLN) | 14 | 201 |
a
The vSGLT OF conformation was generated from the X-ray structure of the homolog SiaT.