Fig. 3. DCIP and cyt c reduction assays.

a DCIP reduction activities of the three redoxin reductases in the presence of 1 mM NADH or NADPH. b Rates of electron transfer measured by cyt c reduction. The reaction mixture contained 30 μM cyt c, 10 nM FdR or 10 nM FdR coupled with 20 nM Fdx, and started by adding 500 μM NAD(P)H. All experiments were independently repeated for three times. The error line represents the standard deviation. P values in each groups were calculated with single factor ANOVA analysis, and all P values were <0.01.