Fig. 5. Neutrophils release mitochondrion-containing EVs during migration and reduce endothelial ROS accumulation.
a–b Representative images from intravital imaging of neutrophils (Ly6G-Red, red arrows) within liver vasculature (Dextran-Green) in wild type mice. The white arrow indicates a vesicle released by a migrating neutrophil. Scale bar in a, 30 μm; Scale bar in b, 5 μm. c–d Representative images from intravital imaging of neutrophils (Ly6G-Red) within liver vasculature (CD144-Blue) in PhAMfloxed × Mrp8-Cre mice. Mitochondria of neutrophils were labeled by Mito-Dentra2 (Dendra2-Green). The white arrow indicates a mitochondrion-containing vesicle released by a migrating neutrophil. Scale bar in (c), 15 μm; Scale bar in d, 5 μm. e Flow analysis of ROS levels in hepatic endothelial cells 1 h after PBS or lethal LPS in the indicated recipient mice. f M.F.I. of ROS levels in hepatic endothelial cells 1 h after PBS or lethal LPS in the indicated recipient mice (n = 5 per group). Source data are provided as a Source Data file. Data are representative of or pooled from at least three independent experiments. Data are mean ± SD. Two-tailed unpaired t tests were used for statistical analyses. *p < 0.05, **p < 0.01, ***p < 0.001.