Cytokine and chemokine levels in lung allografts.

(A) MIP-1α, MIP-1β and RANTES levels in lung digests of resting B6 lungs or Balb/c to B6 lung grafts with or without CSB immunosuppression by Multiplex Assays. (B) Relative expression of eotaxin-1, eotaxin-2 or IL-5 in lung grafts by quantitative RT-PCR. (C) IL-5 production in Balb/c to B6 lung grafts with or without immunosuppression by flow cytometric evaluation of lung digests. (D) Recipient-derived (top) or adoptively transferred CD45 congenic eosinophils (bottom) in the left lung graft in the presence of IL-5 neutralization or Control IgG treatment. (E) Tissue infiltration of adoptively transferred eosinophils in the left lung graft of Balb/c to B6 recipients after IL-5 neutralization or Control IgG treatment. Representative of two separate experiments. (F) Eosinophil transwell migration in the presence or absence of eotaxin-1 and graded concentrations of IL-5. For (A) to (C), lung tissue was collected and analyzed at post-engraftment day 4. For (D) and (E), eosinophils were injected at post-engraftment day 4 and the tissue was collected 18 hours later. CSB=co-stimulatory blockade, ns=p>0.05; * =p<0.05; **=p<.01; ***=p<.001