A-C, Original recording traces (A), cumulative probability plots (B), and quantification (C) show the effects of AP5 on the frequency and amplitude of mEPSCs of spinally projecting PVN neurons of rats 7 days after discontinuing treatment with FK506 or vehicle (n = 8 neurons from 3 rats per group). D and E, Representative recording traces and quantification show puff-elicited NMDA currents (D, n = 8 neurons from 3 rats in the vehicle group, n = 9 neuros from 3 rats in FK506 group) and puff-elicited AMPA currents (E, n = 10 neurons from 4 rats per group) of spinally projecting PVN neurons of rats 7 days after discontinuing treatment with FK506 or vehicle. C, two-way ANOVA followed by Bonferroni’s post hoc test; D and E, two-tailed Student’s t test. Data are expressed as mean ± SEM.