A-D, Original recording traces (A and B) and quantification (C and D) show the effects of microinjection of CNQX into the PVN on mean arterial blood pressure (MAP), heart rate (HR), and integrated renal sympathetic activity (Int-RSNA) in rats 7 days after discontinuing treatment with FK506 or vehicle (n = 7 rats per group). Repeated measures ANOVA followed by Dunnett’s post hoc test Data are expressed as mean ± SEM. E, Representative brightfield and fluorescence images and schematic drawings show the CNQX microinjection sites in the PVN in vehicle-treated (○) and FK506-treated rats (●). Scale bar, 1 mm. 3V, third ventricle; OX, optic chiasm; AHA, anterior hypothalamic area; LA, latero-anterior hypothalamus; AHC, central division of the anterior hypothalamus.