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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Alcohol Clin Exp Res. 2022 Jun 8;46(7):1321–1330. doi: 10.1111/acer.14876

Figure 4. Differences in fron-tloading as a function of diet and previous experience with ethanol.

Figure 4.

(A) Animals were maintained on one of two diets (TL20 or LD01) during the first DID session (D1-D15), and then were switched to the opposite diet for a period of two weeks before undergoing a second DID session (D16-D30). Cumulative consumption over the 2 hour DID, dotted line indicates the first 15 min period within DID, on Day 1 (B) and D15 (C) demonstrates elevated ethanol front-loading in mice maintained on the LD01 diet compared to mice on the TL20 diet. (D) Differences in ethanol front-loading (first 15 minutes) emerged between LD01 and TL20 by day 4, and persisted through the first 15 days. (E) Mice were switched to the opposite diet for a period of 2 weeks before DID was conducted for a second block and cumulative consumption was measured over the 2 hour DID on Day 16 (E) and Day 30 (F). (G) Differences in ethanol front-loading (first 15 minutes) over block 2 (D16-D30) but overall ethanol consumption increased, especially in the mice switched from TL20 to LD01.