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. Author manuscript; available in PMC: 2022 Aug 7.
Published in final edited form as: Science. 2022 Mar 17;375(6586):1287–1291. doi: 10.1126/science.abm3282

Fig. 1. Structural characterization of M. capsulatus (Bath) pMMO in a native lipid nanodisc.

Fig. 1.

(A) Methane oxidation activity of cryo-EM samples. Addition of 3 equivalents of CuSO4 during nanodisc (ND) reconstitution (+ Cu) improved activity. Error bars represent standard deviations of n ≥ 3 biological replicates, each measured in triplicate. (B) Cryo-EM map (dataset MC01) showing the trimer and the encircling nanodisc. Symmetrical αβγ protomers are colored in purple, teal, and gold, respectively.(C) Model of pMMO showing the map for lipids on the periphery and in the inner pore of the enzyme. The periplasmic and cytoplasmic leaflets are colored in pink and blue, respectively.