TABLE 3.

Ability of BglG derivatives to antiterminate transcription of a chromosomal bgl-lacZ fusion^a

Plasmid	Plasmid-encoded BglG derivativeb	β-Galactosidase activityc
pMN25		55 ± 5
pAW25		19 ± 4
pAB62		3 ± 1
pAB104d		68 ± 4
pAB33d		2 ± 2
pAB70d		3 ± 2
pANS104m3d		3 ± 3

^aThe experiment was performed with E. coli MA152 strain which has bgl deleted and carries a bgl-lacZ transcription fusion (40). Cells were grown in minimal medium containing 0.4% succinate. 

^bThe numbers of amino acids of BglG are indicated. The broken lines indicate deleted regions in BglG. L, leucine; R, arginine; A, alanine. 

^cThe values (in Miller units) are the means ± standard deviations of four independent measurements.