Fig. 5.

Gadd45 mRNA level is reduced upon dGLYAT depletion. a Volcano plot between dGLYAT RNAi and control groups. Compared with the hs-Gal4 control groups, there are 29 down-regulated genes (indicated by blue points) and 13 up-regulated genes (showed by red points) in the hs > dGLYAT-IR groups, when log₂FC > 1.0 or <-1.0 and adjusted P value < 0.001. Grey dotted lines show thresholds of log₂FC (1.0 and − 1.0) and adjusted P value (0.001). b Heat map of 29 down-regulated genes between dGLYAT RNAi (R1-R6) and control (W1-W6) groups when log₂FC<-1.0 and adjusted P value < 0.001. Genes showing significant changes are listed in Additional file 1: Table S1. c RT-qPCR data showed that Gadd45 mRNA level was decreased upon dGLYAT depletion. *P < 0.05, **P < 0.01. Third instar larvae were heat shocked at 37℃ for 30 min and recovered at 29℃ for 2 h (c) or 6 h (a-c) prior to tissues dissection and RNA extraction