TABLE 1.

Genetic stability assessment

	Method	Advantages	Disadvantages
Cells	Karyotyping	Whole‐genome analysis, Detection of aneuploidy, polyploidy, and other large chromosomal imbalances	Time consuming, Small resolution (High number of metaphases are needed), Cannot detect subkaryotypic variants
	FISH (fluorescent in situ hybridization)	Karyotype and information about mutations can be obtained	Probes must be known genes/mutations, Limited number of colors can be seen with fluorescent microscope, Not suitable for genome wide application
DNA	Microarray	Provide information on DNA regions with gains or losses	Cannot detect balanced rearrangements such as inversions
	Whole‐genome/exome sequencing	Very high and scalable throughput, High sensitivity and accuracy, Assess the whole genome at single‐base resolution	Expensive, Complex result interpretation
	PCR/ddPCR	High resolution for the CNV and SNV detection, Cost‐effective	Cannot comprehensive screening of chromosomal aberrations

Abbreviations: ddPCR, droplet digital PCR; CNV, copy number variation; SNV, single‐nucleotide variants.