TABLE 3.

Elimination of residual hPSCs

Publication	Strategy	Residual cells	Method	Reference
Tohyama et al.	Metabolic	mESCs, hPSCs	Glucose‐depleted culture medium with abundant lactate	2
Tohyama et al.	Metabolic	hPSCs	Glucose‐ and glutamine‐depleted culture medium with abundant lactate	3
Ben‐David et al.	Metabolic	hPSCs	Oleate synthesis inhibition	5
Tanosaki et al.	Metabolic	hPSCs	Fatty acid synthesis inhibition	7
Shiraki et al.	Metabolic	hPSCs	Methionine‐free culture medium	37
Ben‐David et al.	Immunological	hPSCs	Claudin 6‐targeted selection	60
Okada et al.	Immunological	hPSCs	GPC3 reactive cytotoxic T lymphocyte‐based selection	61
Sougawa et al.	Immunological	hiPSCs	Brentuximab vedotin inducible cytotoxicity	62
Schriebl et al.	Immunological	hESCs	hESC‐specific antibodies based selection	63
Nagashima et al.	Biophysical	hiPSCs	Treatment with high concentration of L‐alanine	64
Cho et al.	Biophysical	mESCs (Insertion of suicide gene)	Phototoxicity	65
Kim et al.	Biochemical	hiPSCs	BV treatment	66
Kuang et al.	Biochemical	hPSCs	D‐3 treatment	67
Matsumoto et al.	Biochemical	hiPSCs	Plasma‐activated medium induced selective cell death	68
Tateno et al.	Biochemical	hPSCs	Lectin‐toxin fusion protein	69
Burkert et al.	Biochemical	hPSCs	Salicylic diamine treatment	70
Wu et al.	Genetic	hPSCs (In‐frame iC9 gene insertion into the SOX2 locus to target undifferentiated hESCs)	Induction of apoptosis by iC9 inducer AP1903	71
Elovic et al.	Genetic	hESCs (miR‐499‐responsive lethal mRNA is designed)	Induction of apoptosis by miR‐499‐responsive lethal mRNA	72
Parr et al.	Genetic	hPSCs (miRNA switch [miR‐302/367] is encoded)	Puromycin selection	73
Blum et al.	Genetic	hESCs (A plasmid containing the dominant negative survivin isoform fused in frame to GFP is transfected)	Genetic and pharmacological ablation of survivin	74
Martin et al.	Genetic	hPSCs (NANOG iC9‐YFP and ACTB OiC9‐mPlum /ACTB TK‐mPlum are introduced)	Drug inducible selection	75

Abbreviations: BV, Ban venom; GPC3, Glypican‐3; iC9, Inducible caspase‐9; OiC9, Orthogonal inducible caspase‐9; TK, thymidine kinase.