KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| LRP6 | Abcam | Cat#134146 |
| BRACHYURY | Santa Cruz | Cat#20109; RRID:AB_2255702 |
| Beta-CATENIN | Cell Signaling | Cat#8480; RRID:AB_11127855 |
| SLUG | Cell Signaling | Cat#9585; RRID:AB_2239535 |
| OCT4 | Santa Cruz | Cat#5279; RRID:AB_628051 |
| SOX2 | Abcam | Cat#97959; RRID:AB_2341193 |
| NANOG | Abcam | Cat#62734; RRID:AB_956161 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Matrigel | Corning | Cat#354277 |
| Y-27632 | Selleckchem | Cat#S1049 |
| CHIR99021 | Stemgent | Cat#04-0004 |
| Wnt3a recombinant protein | StemRD | Cat#w3a-h-005 |
| Deposited Data | ||
| Design files, BOM, and code | GitHub | https://github.com/LAVAboard/LAVA |
| Experimental Models: Cell Lines | ||
| H9 Human Embryonic Stem Cells | WiCell | N/A |
| Software and Algorithms | ||
| LAVA graphical user interface | GitHub | https://github.com/LAVAboard/LAVA |