Extended Data Table 1 |.
Study | Tissue/cell | Method | sSNV increase per year per cell | Mutational signatures of aging cells |
---|---|---|---|---|
Blokzijl et al. 20166 | adult stem cells of small intestine, colon, liver | WGS of clonal organoid cultures derived from primary multipotent cells | 35–40 | COSMIC Signature 5 |
Hoang et al. 201676 | bulk brain (frontal cortex), colon, kidney | dilution followed by WGS (BotSeqS) | ~33 (in bulk brain) | |
Lodato et al. 20185 | neurons (prefrontal cortex) | single-cell WGS | ~23 | COSMIC Signature 5 |
Osorio et al. 20187 | hematopoietic stem cells | WGS on clonal cultures | 14 | COSMIC Signature 5 |
Franco et al. 201877 | skeletal muscle resident progenitor/stem (satellite) cells | WGS on in vitro clonally expanded single cells | 13 | COSMIC Signatures 1,5,8 |
Zhang et al. 201978 | B lymphocytes | single cell WGS | ~26 | COSMIC Signatures 1,5 |
Lee-Six et al. 201979 | colon (crypts) | WGS of colorectal crypts, to represent clones from colorectal stem cells | >40 | COSMIC SBS5, SBS1 |
Franco et al. 201980 | kidney tubules, epidermis, subcutaneous adipose, visceral adipose | WGS on in vitro clonally expanded single cells | ~55 (KT2) ~12 (KT1) ~20 (adipose) |
COSMIC SBS1, SBS3/8, SBS5, SBS40 |
This study | neurons (prefrontal cortex and CA1 hippocampus) | single-cell WGS | 16–21 | COSMIC SBS5 |
Of note, COSMIC v3 single base substitution signatures SBS1 and SBS5 are similar and analogous to v2 signatures 1 and 5, respectively (https://cancer.sanger.ac.uk/cosmic/signatures). The table refers to several previous studies.