TABLE 2.
PCR amplification primers used in this study
| Primer | Sequence (restriction enzyme)a |
|---|---|
| B. amyloliquefaciens amyQ forward | CGCCATGGTTCAAAAACGAAAGC (NcoI) |
| B. amyloliquefaciens amyQ reverse | GCGGATCCTTATTTCTGAACATA (BamHI) |
| B. subtilis yvaL forward | AAAGGATCCTAGTCTGGAGGTGTATGGGATGC (BamHI) |
| B. subtilis yvaL reverse | AAATCTAGATTCTCGAGCCCTATAGGATATAAGCAAGC (XbaI) |
| E. coli secG forward | CCCGGATCCGGAGGTTTTAATTCATGTATGAAGCTCTTT (BamHI) |
| E. coli secG reverse | CCCTCTAGACTCGAGTTAGTTCGGGATATCGC (XbaI) |
a
Recognition sites of the restriction enzymes used are underlined. Ribosome binding sites and start and stop codons are in boldface.