TABLE 3.
Strain | Expression | Growtha
at:
|
||
---|---|---|---|---|
15°C | 20°C | 25°C | ||
DB104::111 | ++ | ++ | ++ | |
DB104::470 | E. coli SecG | ± | ± | ++ |
DB104::471 | B. subtilis YvaL | ++ | ++ | ++ |
ΔyvaL::111 | ± | ± | ++ | |
ΔyvaL::470 | E. coli SecG | − | − | ++ |
ΔyvaL::471 | B. subtilis YvaL | ++ | ++ | ++ |
DB104::468 | α-Amylase | ++ | ++ | ++ |
DB104::472 | α-Amylase, E. coli SecG | ± | ± | ++ |
DB104::473 | α-Amylase, B. subtilis YvaL | ++ | ++ | ++ |
ΔyvaL::468 | α-Amylase | − | − | ++ |
ΔyvaL::472 | α-Amylase, E. coli SecG | ± | ± | ± |
ΔyvaL::473 | α-Amylase, B. subtilis YvaL | ± | ± | ± |
Growth was scored as follows: ++, growth like that of the reference; ±, growth slower than that of the reference; −, no growth.