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. 2022 Jul 19;26(16):4492–4505. doi: 10.1111/jcmm.17474

FIGURE 4.

FIGURE 4

MgIG reduced CRIZO‐induced ROS via upregulation of respiratory electron transport chain activity and Nrf2/HO‐1 Pathway. (A) HL‐7702 cells were treated with 15 μM CRIZO or 1 mg/ml MgIG alone or in combination for 24 h. The levels of ROS were determined by flow cytometric analysis (n = 3). (B) The expression levels of HO‐1 and Nrf2 in whole cell and Nrf2 in nucleus were determined by Western blotting (n = 3). (C) The transcription levels of HO‐1 and Nrf2 were determined by qRT‐PCR (n = 3). (D) The activity of RCC Ι was determined by Mitochondrial Respiratory Chain Complexes Ι Activity Assay Kits (n = 3). The data are expressed as the mean ± SD; **p < 0.01, ***p < 0.001 and ****p < 0.0001 vs. vehicle group; # p < 0.05, ## p < 0.01, ### p < 0.001 and #### p < 0.0001 vs. CRIZO 15 μM treatment group.