Fig. 2.

Loss of CSE expression exacerbates acute inflammation and reduces epithelial proliferation. A) Densitometric analyses of western blots comparing CSE and CBS protein levels in colonic mucosa scrapings from CSE-KO (n = 9) and WT (n = 9) mice. (D'Agostino & Pearson Normality test, Unpaired t-test, two-tailed, bars represent mean ± SD, p values shown). B) Effects of dextran sulfate sodium salt (DSS) exposure (7 days) and dl-progargylglycine (PAG) treatment on mouse body weight, and C) colonic mucosa H₂S production. D) Effects of DSS and the exogenous H₂S donor GYY4137 (GYY) on colon inflammation. Statistical analyses of data in panels B-D: Shapiro-Wilk Normality test, one-way ANOVA, Tukey multiple comparisons, p values shown. E) Quantification of Ki67-positive cell nuclei per colonic crypt from WT and CSE-KO mice following exposure to DSS drinking water for 7 days (Two-way ANOVA, Tukey multiple comparisons). F) Representative images of colon tissue sections from DSS-treated WT and DSS-treated CSE-KO mice immunostained for the proliferation antigen Ki67 (brown) (200× magnification). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)