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. 2022 Jul 21;55:102417. doi: 10.1016/j.redox.2022.102417

Fig. 4.

Fig. 4

CSE regulates cellular processes involved in epithelium restitution. A) Effect of PAG-mediated CSE inhibition on basal HCEC migration. B) Effects of siRNA-mediated downregulation of CSE expression on basal HCEC migration (siCSE-1 sense: 5′-GCAUCUGAAUUUGGAUUAA-3’; anti-sense: 5′-UUAAUCCAAAUUCAGAUGC-3′, Ambion Life Technologies, ThermoFisher Scientific, USA). C) Effects of PAG-mediated CSE inhibition on basal and TNF-α-stimulated (10 ng/ml) HCEC migration. D) Effects of PAG-mediated CSE inhibition on basal and IL-10-stimulated (10 ng/ml) HCEC migration. Statistical analyses of data in panel A–D: Shapiro-Wilk Normality test, one-way ANOVA, Tukey multiple comparisons, p values shown). E) Effects of PAG (3 mM) on GYY (0.4 mM) and TNF-α (1 ng/ml) regulation of TEER. Gray band between time −2 and 0 h indicates the time-period of PAG pretreatment. TNF-α and/or GYY was added to cultures at time = 0 (doted-line). Statistical analysis of data in panel E: 2-way ANOVA, Tukey multiple comparison test, *p < 0.0001 [Vehicle vs. TNF-α or GYY], †p < 0.01 [PAG + GYY vs. GYY], ‡p < 0.0001 [PAG or PAG + TNF-α vs. PAG vs. PAG + GYY or PAG + TNF-α & GYY].