Fig. 5.

Loss of CSE accelerates the development of CAC in AOM/DSS-treated mice. A) Timeline of the AOM/DSS colitis-associated carcinogenesis experiment. Mice received up to three exposures (cycles) of drinking water containing DSS. Arrows indicate when mice received an injection of the mutagen azoxymethane (AOM) and the days when colon tissue was harvested to assess tumor burden. B) Example images of a CSE-KO colon harvested on day 63. Asterisks on the top image indicate individual tumors. The percentage of tumor burden (area) was calculated by dividing the sum of the areas of all the individual tumors (outlined in black in the bottom image) by the area of the total colon (also outlined in black) using Image J software. C&D) Box and Whisker plots comparing the effects of AOM and DSS treatment of WT and CSE-KO mice on tumor number at Day 21 C) and tumor burden (i.e., % Tumor Area/Colon) at Days 42, 63, and 80 D) (Mann-Whitney test, two-tailed, exact p values shown, +: mean).