TABLE 5.

Effect of an AUG repA start codon on expression from repA-lacZ fusions

Mutation present in repA-lacZ fusion	β-Galactosidase activity (U) with coresident plasmid present in transa:
	Vector (pACYC177)	RNAI (pMU3265)	Target (pMU3268)
None	120 ± 7	0.2	990 ± 101
AST2	352 ± 50	44 ± 3	2,347 ± 192
AST2 + G671C	141 ± 34	25 ± 1	787 ± 52
AST2 + BTER−38	33 ± 3	6 ± 0.6	89 ± 9
AST2 + G671C + BTER−38	0.8	1	0.7
BTER−38	0.3	<0.1	2 ± 0.5
BST2b	4 ± 1	<0.1	28 ± 4
AST2 + BST2	87 ± 7	8 ± 0.7	299 ± 30

^aSee Table 2, footnote a. 

^bThe BST2 (ATG→ACG) mutation has been described previously (5).