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qRT-PCR experiment to verify the expression of PRGs of interest in the in vitro model. HRECs exposed to high glucose for 48 h were used as DR model, and HRECs cultured on normal culture medium were used as control. The P-values were calculated using a two-sided unpaired Student’s t-test. *P < 0.05; **P < 0.01. qRT-PCR, quantitative real-time polymerase chain reaction; PRGs, pyroptosis-related genes; HRECs, human retinal endothelial cells; DR, diabetic retinopathy.
