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. 2022 Jul 25;13:918605. doi: 10.3389/fendo.2022.918605

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Copyright © 2022 Wang, Ding, Liu, Zhang, Zheng, Xia and Xiong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analysis of caspase-3 and gasdermin proteins in in vitro models. (A) Cleaved caspase-3 (17 kDa) was tested. GAPDH (36 kDa) was used as a control. (B) GSDMD (31 kDa) was examined. GAPDH was used as a control. (C) GSDME (55 kDa) was examined. GAPDH was used as a control. The experiments were repeated independently at least three times. *P < 0.05. CON, control group; HG, high-glucose group; GSDMD, gasdermin D; GSDME, gasdermin E.